DNA vaccination may represent an interesting strategy for early life immunization. However, in some cases, this approach has been shown to induce a tolerance rather than immunity. We have compared the efficiency of neonatal DNA or protein immunization against hepadnavirus envelope protein using the duck hepatitis B virus (DHBV) model. Three-day-old ducklings were immunized with either a plasmid encoding the DHBV pre-S/S large envelope protein (L), or a recombinant preS protein, followed by sequential DNA or protein boosts at weeks 4 and 15. Our results showed that genetic immunization of duck neonates induced specific humoral response to DHBV L protein. Interestingly, an enhanced antibody response was elicited when animals received DNA priming-DNA boosting as compared to DNA priming-protein boosting.

Type

Journal article

Journal

Vaccine

Publication Date

15/07/2000

Volume

18

Pages

3091 - 3096

Keywords

Animals, Animals, Newborn, Ducks, Hepatitis Antibodies, Hepatitis B Virus, Duck, Immunization, Immunization Schedule, Vaccines, DNA, Viral Envelope Proteins, Viral Vaccines